منابع مشابه
Synchrotron Microangiography of the Rat Heart Using the Langendorff Model
Background and Objectives: The ability to study microvessels of a beating heart in real time at the level of the capillary is essential for research. However, there are no proven methods currently available to achieve this. The conventional absorption-contrast agents have limitations for studying capillaries. Microangiography with using synchrotron phase-contrast X-ray technology and no contras...
متن کاملHigh-resolution Ex-vivo Mr Angiography of the Murine Heart Using Langendorff Gd-dtpa Perfusion Technique
Methods: Hearts were excised from normal mice and from a genetically modified mouse model developed to study cardiac hypertrophy [3]. Animals were humanely killed by cervical dislocation, the aorta cannulated and retrogradely perfused at constant flow (~ 3ml/min) using a Langendorff perfusion system. A first solution containing 0.75mM CaCl2 was perfused to allow the hearts to contract and clear...
متن کاملControlled regional hypoperfusion in Langendorff heart preparations.
We describe a new approach that combines several techniques to allow abnormal electrical and calcium activity to be visualized within hypoperfused myocardial tissue. A flexible microcannula was inserted into the left anterior descending artery of Langendorff perfused rat hearts, an air-tight seal between the coronary artery and the cannula was created, and an HPLC pump was used to deliver a spe...
متن کاملA novel isolated dual perfusion/superfusion heart model for physiological and pharmacological evaluation of mammalian heart preparations
Abstract Introduction: Isolated perfused heart models such as perfusion and superfusion are commonly used for mammalian heart research. However, there are several fundamental limitations in the current techniques. In perfusion model, a suitable cannula is connected to the aorta and the perfusion is retrogradely performed. But, electrode displacement is a potential unwanted event resulted fr...
متن کاملThe alpha(1B)-adrenergic receptor decreases the inotropic response in the mouse Langendorff heart model.
OBJECTIVE alpha(1)-Adrenergic receptors (ARs) are known mediators of a positive inotropy in the heart, which may play even more important roles in heart disease. Due to a lack of sufficiently selective ligands, the contribution of each of the three alpha(1)-AR subtypes (alpha(1A), alpha(1B) and alpha(1D)) to cardiac function is not clearly defined. In this study, we used a systemically expressi...
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ژورنال
عنوان ژورنال: Folia Pharmacologica Japonica
سال: 2012
ISSN: 0015-5691,1347-8397
DOI: 10.1254/fpj.140.166